New Insight of Methylenetetrahydrofolate Reductase (MTHFR) C677T Gene Polymorphisms, and Serum Electrolytes in Cardiac Syndrome X Patients

Background: Cardiac Syndrome X (CSX) is a condition affecting the cardiovascular system with significant degree of morbidity. Diagnosis and treatment are challenging when cause unclear. Subsequently, molecular marker for screening people CSX highly recommended. The present study evaluated association between MTHFR C677T gene polymorphism among Sudanese patients CSX.
 Materials Methods: A total 100 subjects were enrolled. Venous blood sample was collected from each participant in Ethylene Diamine Tetracetic Acid (EDTA) containers. DNA extracted samples using guanidine chloride method mutation detected by PCR-restriction fragment (PCR-RFLP). Statistical package social sciences (SPSS) used to analyze data.
 Results: Most 30(60%) females, their age ranged 30-60 mean 44.98±7.34 SD. 677CT genotype frequency statistically (P≤0.014), where 10(20%) had 1(2%) 677TT group respectively compared control individuals who only 2(4%) 677CT. T alleles significantly more frequent our than C alleles. There insignificant slightly decreased (2.4 ±2.8, 2.5±3.2) serum magnesium levels respectively, as well random glucose. Elevated cholesterol, HDL (182 ±18.1, 49.7±7.1) vs (180 ±20.3, 46.6 ±11.3) group, all findings non-significant. Slightly decrease level (2.2 ±2.1, 2.9 ±0.8) heterozygous CT genotypes homozygous genotypes.
 Conclusion: linked population, C677T. Furthermore, could be disease technique.